Recombinant Human ABL1 Protein, GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: ABL1-783HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human ABL1 (NP_009297.2) amino acid sequence (Pro 137-Ser 554), fused with the GST tag at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 645
• Form: Lyophilized
• Molecular Mass: The recombinant human ABL1/GST chimera consists of 645 amino acids and predicts a molecular mass of 74 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM EDTA, 0.5 mM Reduced Glutathione, pH 8.0
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: ABL1 c-abl oncogene 1, non-receptor tyrosine kinase [ Homo sapiens ]
• Official Symbol: ABL1
• Gene ID: 25
• mRNA Refseq: NM_005157
• Protein Refseq: NP_005148
• MIM: 189980
• UniProt ID: P00519