Active Recombinant Saccharomyces Cerevisiae ICDH protein

• Cat.No.: ICDH-81
• Product Overview: Recombinant full length Saccharomyces Cerevisiae ICDH (NADP) was expressed in Yeast. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression.

Specification

• Species: S.cerevisiae
• Source: Yeast
• Tag: Non
• Description: Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
• Form: One ml of solution (1mg/282ul)contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.
• Bio-activity: The specific activity was found to be 115 U/mg.
• Purity: Greater than 95.0% as determined by:
  (a) Analysis by RP-HPLC.
  (b) Analysis by SDS-PAGE.
• Unit Definition: One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25 centigrade, pH 7.5).
• Storage: Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time.
  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  Avoid multiple freeze-thaw cycles.
• Full Length: Full L.