| Cat.No.: | ICDH-81 |
| Product Overview: | Recombinant full length Saccharomyces Cerevisiae ICDH (NADP) was expressed in Yeast. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression. |
| Description: | Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. |
| Source: | Yeast |
| Species: | Saccharomyces Cerevisiae |
| Form: | One ml of solution (1mg/282ul)contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1. |
| Bio-activity: | The specific activity was found to be 115 U/mg. |
| Purity: | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Unit Definition: | One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25 centigrade, pH 7.5). |
| Storage: | Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
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