|Product Overview:||Recombinant full length Saccharomyces Cerevisiae ICDH (NADP) was expressed in Yeast. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression.|
|Description:||Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.|
|Form:||One ml of solution (1mg/282ul)contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1.|
|Bio-activity:||The specific activity was found to be 115 U/mg.|
|Purity:||Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
|Unit Definition:||One unit is defined as 1 µmol of NAD+ production per minute under the assay conditions (25 centigrade, pH 7.5).|
|Storage:||Store at 4 centigrade if entire vial will be used within 2-4 weeks. Store, frozen at -20 centigrade for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.