Cat.No.: | Skeletal Muscle-5H |
Product Overview: | Creative Biomart's membrane proteins are prepared from a large variety of documented and compliant quality tissues, including normal and diseased tissues from human and other species. |
Description: | Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.? |
Species: | Human |
Concentration: | 2 mg/ml |
Components: | All components may be considered bio-hazardous and may contain unidentified contagious material. |
Shipping: | Dry Ice |
Quality control Testing: | 1. Protein lysate is verified on SDS-PAGE analysis as a consistent pattern. 2. Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein. |
Safety Testing: | Effects of Overexposure: N/A Respiratory Protection: Wear masks while handling protein sample. Ventilation: General ventilation. Fume hoods should maintain a minimum face velocity of 100 lfm. Protective Glove: General lab safe impermeable gloves Eye Protection: Wear splash goggles or full-face shield. Keep eyewash station in working order. Handling and Storage: Wear appropriate protective clothing and avoid using sharp material such as needle, blade or broken glasses. Store in cold.Environment Protection: Treat all lab supplies that have contaminated with protein product with 2% bleach for 30 minutes, then autoclave for reusing or through away as biohazards. |
Storage Buffer: | HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent and a cocktail of protease inhibitors |
Storage Instruction: | See User's Manual for storage information. Handling and use as infectious material. Work under fume hood. Protect workers and environment. Store product in a safe place. Avoid unauthorized access. |
Applications: | Western Blot (10 μg to 20 μg per lane is recommended for a mini gel). Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, tissue specific expression. |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.