Cat. No.:	DnaK-316
Product Overview:	Recombinant DNAK was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques (132 aa, 14.6 kDa). Additional amino acid(Met) is attached at N- terminus.
Description:	DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak(residues 508-638) of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft.
Source:	E.coli.
Formulation:	Liquid. In 25 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 1 mM DTT, 10% glycerol.
Molecular Weight:	14.6 kDa (132aa).
Purity:	> 95% by SDS – PAGE.
Concentration:	1 mg/ml (determined by Bradford assay).
Sequence:	MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK
Storage:	Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Name:	dnaK
Synonyms:	dnaK; dnaK chaperone Hsp70, co-chaperone with DnaJ; Hsp70 protein; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg; chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins.
Gene ID:	944750
Protein Refseq:	NP_414555.1
UniProt ID:	P0A6Y8

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