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Recombinant Human Peptidylprolyl Cis/trans Isomerase, NIMA-interacting 1

Cat. No.: PIN1-351H
Product Overview: Recombinant human PIN1 protein expressed in E.coli, 18.2 kDa (163 aa).
Description: Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
Source: Escherichia Coli.
Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 5 mM DTT, 20% glycerol.
Purity: > 95% by SDS - PAGE.
Concentration: 1 mg/ml (determined by Bradford assay)
Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
Sequences of amino acids: MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNG QG E PARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELINGYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSG PV FTDSGIHIIL RTE
Biological activity: Specific activity is > 330 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
Activity Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant PIN 1 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: PIN1 peptidylprolyl cis/trans isomerase, NIMA-interacting 1 [ Homo sapiens ]
Synonyms: PIN1; DOD; UBL5; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; prolyl isomerase; peptidyl-prolyl cis/trans isomerase, NIMA-interacting; protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1; EC 5.2.1.8; dod; PPIase Pin1; Rotamase Pin1; peptidyl-prolyl cis/trans isomerase, NIMA-interacting 2; prolyl isomerase 2; protein (peptidyl-prolyl cis/trans isomerase); NIMA-interacting 1; protein (peptidylprolyl cis/trans isomerase); NIMA-interacting 1.
Gene ID: 5300
mRNA Refseq: NM_000143
Protein Refseq: NP_000134
MIM: 136850
UniProt ID: P07954
Chromosome Location: 1q42.1
Function: fumarate hydratase activity, fumarate hydratase activity, lyase activity

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