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Recombinant Mouse Slit3, His-tagged

Cat.No.: Slit3-123M
Product Overview: Recombinant Mouse Slit3(Ser27-His901) fussed with 10-His tag at C-terminal was expressed in Mouse myeloma cell line, NS0.
Description: Slit3 is a member of the slit family of large secreted axon guidance molecules that are ligands for Robo receptors. Like other mammalian family members, the 1523 amino acid (aa), 200 kDa Slit3 contains a signal sequence followed by 23 leucine­rich repeats (LRR) and nine EGF­like sequences. Mammalian Slits also contain a laminin­G domain between EGF6 and EGF7, and a C­terminal cysteine­rich domain. In Drosophila Slit, specific LRR are sites of ROBO interaction and homodimerization. Drosophila Slit shows equal similarities with all three mouse slit proteins, which are 59­66% identical with each other. During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2. Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in function. Slit3 is also expressed in the lung, kidney, skeletal muscle and heart, both during development and postnatally. ROBO2 is often found in complementary areas. Mice with genetically disrupted Slit3 have thin diaphragms with disorganized collagen fibrils and frequently develop diaphragmatic hernias. Abnormalities in kidney development may also occur. Although Slit proteins are generally considered to be secreted, significant amounts of Slit3 may be retained in the mitochondria because of features in the signal sequence that indicate a high probability of mitochondrial targeting. Secreted Slit is often membrane­associated. Mouse Slit3 shows 98% and 94% amino acid identity with rat and human Slit3, respectively.
Source: NS0 cell
Species: Mouse
Tag: His
Predicted N Terminal: Ser27
Form: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl. See Certificate of Analysis for details.
Bio-activity: Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons.Able to significantly enhance neurite outgrowth when immobilized at 6-12 µg/mL.
Molecular Mass: Predicted Molecular Mass:99.1 kDa; SDS-PAGE:115-130 kDa, reducing conditions
Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze­thaw cycles.12 months from date of receipt, ­- 20 to ­- 70 centigrade as supplied;1 month, 2 to 8 centigrade under sterile conditions after reconstitution;3 months, ­- 20 to ­- 70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.
Gene Name: Slit3 slit homolog 3 (Drosophila) [ Mus musculus ]
Official Symbol: Slit3
Synonyms: Slil2; Slit1; b2b2362.1Clo; slit homolog 3 protein; slit-3
Gene ID: 20564
mRNA Refseq: NM_011412
Protein Refseq: NP_035542
UniProt ID: Q9WVB4
Chromosome Location: 11; 11 A5
Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem
Function: Roundabout binding; calcium ion binding; receptor binding

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