"RNase H" Related Products

Recombinant RNase H protein

Cat.No.: RNASEH1-433H
Product Overview: Recombinant RNase H was expressed in E. coli.
Description: Ribonuclease H (RNase H) is an RNA-specific endonuclease that hydrolyzes single-stranded RNA via a magnesium or manganese cofactor. As such, RNase H is often used to remove the RNA template following reverse transcription or cDNA generation.
Source: E. coli
Form: Ribonuclease H (RNase H) is supplied in a buffer of 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol.
Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate.
Applications: Removal of RNA template following second-strand cDNA synthesis;Removal of mRNA poly(A) sequence;Reverse transcription;
Storage: Store at -20 centigrade
Concentration: 20–60 U/µL

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