| Cat.No.: | RNASEH1-433H |
| Product Overview: | Recombinant RNase H was expressed in E. coli. |
| Description: | Ribonuclease H (RNase H) is an RNA-specific endonuclease that hydrolyzes single-stranded RNA via a magnesium or manganese cofactor. As such, RNase H is often used to remove the RNA template following reverse transcription or cDNA generation. |
| Source: | E. coli |
| Form: | Ribonuclease H (RNase H) is supplied in a buffer of 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol. |
| Unit Definition: | One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate. |
| Applications: | Removal of RNA template following second-strand cDNA synthesis;Removal of mRNA poly(A) sequence;Reverse transcription; |
| Storage: | Store at -20 centigrade |
| Concentration: | 20–60 U/µL |
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