Recombinant RNase H protein

• Cat.No.: RNASEH1-433H
• Product Overview: Recombinant RNase H was expressed in E. coli.

Specification

• Description: Ribonuclease H (RNase H) is an RNA-specific endonuclease that hydrolyzes single-stranded RNA via a magnesium or manganese cofactor. As such, RNase H is often used to remove the RNA template following reverse transcription or cDNA generation.
• Source: E. coli
• Form: Ribonuclease H (RNase H) is supplied in a buffer of 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol.
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate.
• Applications: Removal of RNA template following second-strand cDNA synthesis;Removal of mRNA poly(A) sequence;Reverse transcription
• Storage: Store at -20 centigrade
• Concentration: 20–60 U/µL
• Publication:
  Characterization of Escherichia coli RNase H Discrimination of DNA Phosphorothioate Stereoisomers (2021)