|Cat. No. :
|Product Overview :
||Full Length Human HDAC3– Class I, 53kD with his His & GST tag.
||Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
||HDAC3 was cloned from human brain cDNA and expressed in insect cells using a baculoviral expression system.
||Purified, active enzyme.
|Purification Procedure :
||Ni2+ Affinity Column & Glutathione
||1000 units per vial. 1 unit is defined as the amount of enzyme that will convert 1picomole per minute per ug of protein at pH8 at 30°C.
||>6 months from date of shipment
|Buffer Composition :
||25mM Tris pH8, 120mM NaCl, 0.05% Tween-20 & 40% Glycerol.