|Cat. No. :
|Product Overview :
||Recombinant Human Ribosomal Phosphoprotein P0 (His-tagged) produced inSf9is a glycosylated, polypeptide chain. The protein has a molecular mass of 35,096 dalton. The protein is purified by proprietary chromatographic techniques.
||The ribosomal phosphoproteins, also called P protein antigens, are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35 kDa, P1 (19 kDa) and P2 (17 kDa). Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients. If anti-ribosomal P antibodies were to occur in the absence of other typical SLE associated autoantibodies, they may account for some patients with so-called ANA-negative lupus. It has been reported that lupus patients positive for anti-ribosomal P autoantibodies have a high frequency of CNS involvement, suggesting a marker use for these antibodies.
||Sf9 insect cells.
||Recombinant Human Ribosomal Phosphoprotein P0 is supplied at a of 0.9mg/ml in20mM HEPES buffer pH-7.5, 0.01 mM EDTA. 0.02% SDS.
|Immunological Functions :
||1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels.
||Western-Blot with monoclonal anti-hexa-His-tag antibody & SLE sera (Systemic Lupus Erythematodes Disease).
||Greater than 80% (SDS-PAGE).
|Coating Concentration :
||0.3-0.7 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
|Storage Conditions :
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.