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Recombinant Human Peroxiredoxin 5

Cat. No. : PRDX5-181H
Product Overview : Recombinant Peroxiredoxin 5 protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 17.0 kDa (162aa).
Description : Peroxiredoxin 5, also known as PRDX5, is a member of the peroxiredoxin family of antioxidant enzymes, which reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. This protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. It has been reported that peroxiredoxin 5 is involved in intracellular redox signaling.
Source : E.coli.
Amino Acid Sequence : MAPIKVGDAI PAVEVFEGEP GNKVNLAELF KGKKGVLFGV PGAFTPGCSK THLPGFVEQA EALKAKGVQV VACLSVNDAF VTGEWGRAHK AEGKVRLLAD PTGAFGKETD LLLDDSLVSI FGNRRLKRFS MVVQDGIVKA LNVEPDGTGL TCSLAPNIIS QL
Form : Liquid. In 20 mM HEPES buffer (pH 7.4).
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method ).
Biological activity : Specific activity: approximately 117-136 pmole/min/µg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX5 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute.
Activity Assay : 1. Prepare a 50ul reaction mix into a suitable container: the final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol. 2. Add 5 ul of recombinant PRDX5 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer. 3. Incubate at 25°C for 2 minutes. 4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min. 5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN) 7. Record the increase in A475nm.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : PRDX5 peroxiredoxin 5 [ Homo sapiens ]
Synonyms : PRDX5; peroxiredoxin 5; PLP; ACR1; B166; PRXV; PMP20; PRDX6; SBBI10; AOEB166; MGC117264; MGC142283; MGC142285; TPx type VI; Alu co-repressor 1; antioxidant enzyme B166; thioredoxin peroxidase PMP20; liver tissue 2D-page spot 71B; peroxisomal antioxidant enzyme; EC 1.11.1.15; Peroxiredoxin-5, mitochondrial; Prx-V; PLP; Thioredoxin reductase; Thioredoxin peroxidase PMP20; Antioxidant enzyme B166; Alu corepressor 1
Gene ID : 25824
mRNA Refseq : NM_012094
Protein Refseq : NP_036226
MIM : 606583
UniProt ID : P30044
Chromosome Location : 11q13
Function : caspase inhibitor activity; oxidoreductase activity; peroxiredoxin activity

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