| Cat. No. : |
GUCA2A-86H |
| Description : |
Heat-stable enterotoxins (STa) are small, cysteine-rich peptides secreted by Escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as STaR. Binding of STa to STaR induces a dramatic increase in the cGMP content of the cell; the increase, in turn, inhibits salt absorption and stimulates chloride secretion. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to the diarrhea and dehydration characteristic of enterotoxin activity. The hormonal form of guanylin is a 94-amino acid peptide with a molecular mass of 10.3kDa. Guanylin is synthesized by gut enterochromaffin cells as a prohormone of 115 amino acids and is processed to the molecular form of 94 amino acids circulating in the blood. GPVTVQDGNF SFSLESVKKL KDLQEPQEPR VGKLRNFAPI PGEPVVPILC SNPNFPEELK PLCKEPNAQE ILQRLEEIAE DPGTCEICAY AACTGC |
| Source : |
Escherichia Coli. |
| Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation : |
Sterile filtered and lyophilized from 0.5 mg/ml in deionized H2O. |
| Specificity : |
The recombinant human proguanylin is 100% homologous with the human proguanylin. |
| Purification Method : |
Three-step procedure using IMAC and size exclusion chromatography before and after refolding. |
| Solubility : |
Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely. |
| Applications : |
Western blotting. |
| Purity : |
Greater than 95.0% as determined by SDS-PAGE. |
| Stability : |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. |
