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Recombinant Human Guanylate Cyclase Activator 2A

Cat. No. : GUCA2A-86H
Product Overview : Proguanylin Human Recombinant produced inE.coli, is an 11kDa protein containing 96 amino acid residues of the human proguanylin and 2 additional amino acid residues (bold). The Proguanylin is purified by proprietary chromatographic techniques.
Description : Heat-stable enterotoxins (STa) are small, cysteine-rich peptides secreted by Escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as STaR. Binding of STa to STaR induces a dramatic increase in the cGMP content of the cell; the increase, in turn, inhibits salt absorption and stimulates chloride secretion. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to the diarrhea and dehydration characteristic of enterotoxin activity. The hormonal form of guanylin is a 94-amino acid peptide with a molecular mass of 10.3kDa. Guanylin is synthesized by gut enterochromaffin cells as a prohormone of 115 amino acids and is processed to the molecular form of 94 amino acids circulating in the blood. GPVTVQDGNF SFSLESVKKL KDLQEPQEPR VGKLRNFAPI PGEPVVPILC SNPNFPEELK PLCKEPNAQE ILQRLEEIAE DPGTCEICAY AACTGC
Source : Escherichia Coli.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation : Sterile filtered and lyophilized from 0.5 mg/ml in deionized H2O.
Specificity : The recombinant human proguanylin is 100% homologous with the human proguanylin.
Purification Method : Three-step procedure using IMAC and size exclusion chromatography before and after refolding.
Solubility : Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
Applications : Western blotting.
Purity : Greater than 95.0% as determined by SDS-PAGE.
Stability : Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.
Gene Name : GUCA2A guanylate cyclase activator 2A (guanylin) [ Homo sapiens ]
Synonyms : GUCA2A; guanylate cyclase activator 2A (guanylin); GUCA2; STARA; GUANYLIN; Gap-I; Guanylin; Guanylate cyclase activator 2A; Guanylate cyclase-activating protein1; HMW-guanylin; OTTHUMP00000007926; guanylate cyclase activator 2A (guanylin 2, intestinal, heat-stable)
Gene ID : 2980
mRNA Refseq : NM_033553
Protein Refseq : NP_291031
MIM : 139392
UniProt ID : Q02747
Chromosome Location : 1p35-p34
Function : guanylate cyclase activator activity; hormone activity

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