||Recombinant Human Decorin encoding the mature human Decorin (Asp31-Lys359) was recombined with His tag at the N Terminus. That was expressed inE. Coli,and purified by Ni-NTA Column.MW=36.2KDa.
||Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly.
||Mature human Decorin (Asp31-Lys359)31DEASGIGPEV PDDRDFEPSL GPVCPFRCQC HLRVVQCSDL GLDKVPKDLP PDTTLLDLQN NKITEIKDGD FKNLKNLHAL ILVNNKISKV SPGAFTPLVK LERLYLSKNQ LKELPEKMPK TLQELRAHEN EITKVRKVTF NGLNQMIVIE LGTNPLKSSG IENGAFQGMK KLSYIRIADT NITSIPQGLP PSLTELHLDG NKISRVDAAS LKGLNNLAKL GLSFNSISAV DNGSLANTPH LRELHLDNNK LTRVPGGLAE HKYIQVVYLH NNNISVVGSS DFCPPGHNTK KASYSGVSLF SNPVQYWEIQ PSTFRCVYVR SAIQLGNYK 359.
||>95% in SDS gel.
||Lyophilized 100 μg human Decorin in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
||Cell biology, ELISA.
||Add 500 μl deionized water to the vial to prepare a working stock solution at 200 μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
||Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.