Recombinant Human BPGM, His-tagged

• Cat.No.: BPGM-27666TH
• Product Overview: Recombinant full length Human BPGM with a C terminal His tag. 267 amino acids with a predicted MWt 31 kDa including tag.

Specification

• Description: 2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Protein length: 259 amino acids
• Conjugation: HIS
• Molecular Weight: 31.000kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.02% DTT, 10% Glycerol
• Storage: Please see Notes section
• Sequences of amino acids: MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNERHYGALIGLNR EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED QGKVKQAKKL EHHHHHH
• Sequence Similarities: Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Gene Information

• Gene Name: BPGM 2,3-bisphosphoglycerate mutase [ Homo sapiens ]
• Official Symbol: BPGM
• Synonyms: BPGM; 2,3-bisphosphoglycerate mutase; bisphosphoglycerate mutase
• Gene ID: 669
• mRNA Refseq: NM_001724
• Protein Refseq: NP_001715
• MIM: 613896
• Uniprot ID: P07738
• Chromosome Location: 7q31-q34
• Pathway: Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: bisphosphoglycerate 2-phosphatase activity; bisphosphoglycerate mutase activity; hydrolase activity; isomerase activity; phosphoglycerate mutase activity