Recombinant Human EEF2K

• Cat.No.: EEF2K-27779TH
• Product Overview: Recombinant full length human EEF2K with N-terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system; amino acids 1-725, 125kDa.

Specification

• Description: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
• Biological activity: Specific activity of EEF2K was determined to be 50nmol/min/mg as per activity assay protocol
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. Alpha-type protein kinase family.Contains 1 alpha-type protein kinase domain.

Gene Information

• Gene Name: EEF2K eukaryotic elongation factor-2 kinase [ Homo sapiens ]
• Official Symbol: EEF2K
• Synonyms: EEF2K; eukaryotic elongation factor-2 kinase; eukaryotic elongation factor 2 kinase; eEF 2K
• Gene ID: 29904
• mRNA Refseq: NM_013302
• Protein Refseq: NP_037434
• MIM: 606968
• Uniprot ID: O00418
• Chromosome Location: 16p12
• Pathway: IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem; PI3K Cascade, organism-specific biosystem; PKB-mediated events, organism-specific biosystem
• Function: ATP binding; calcium ion binding; calmodulin binding; elongation factor-2 kinase activity; nucleotide binding