Recombinant Human Heat Shock 70kDa Protein 6 (HSP70B), His-tagged

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Recombinant Human Heat Shock 70kDa Protein 6 (HSP70B), His-tagged

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Cat. No. : HSPA6-1811H
Product Overview : Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 73.2 kDa (663aa).
Description : HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress.
Source : E.coli.
Amino Acid Sequence : MGSSHHHHHH SSGLVPRGSH MQAPRELAVG IDLGTTYSCV GVFQQGRVEI LANDQGNRTT PSYVAFTDTE RLVGDAAKSQ AALNPHNTVF DAKRLIGRKF ADTTVQSDMK HWPFRVVSEG GKPKVRVCYR GEDKTFYPEE ISSMVLSKMK ETAEAYLGQP VKHAVITVPA YFNDSQRQAT KDAGAIAGLN VLRIINEPTA AAIAYGLDRR GAGERNVLIF DLGGGTFDVS VLSIDAGVFE VKATAGDTHL GGEDFDNRLV NHFMEEFRRK HGKDLSGNKR ALRRLRTACE RAKRTLSSST QATLEIDSLF EGVDFYTSIT RARFEELCSD LFRSTLEPVE KALRDAKLDK AQIHDVVLVG GSTRIPKVQK LLQDFFNGKE LNKSINPDEA VAYGAAVQAA VLMGDKCEKV QDLLLLDVAP LSLGLETAGG VMTTLIQRNA TIPTKQTQTF TTYSDNQPGV FIQVYEGERA MTKDNNLLGR FELSGIPPAP RGVPQIEVTF DIDANGILSV TATDRSTGKA NKITITNDKG RLSKEEVERM VHEAEQYKAE DEAQRDRVAA KNSLEAHVFH VKGSLQEESL RDKIPEEDRR KMQDKCREVL AWLEHNQLAE KEEYEHQKRE LEQICRPIFS RLYGGPGVPG GSSCGTQARQ GDPSTGPIIE EVD
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 10% glycerol.
Purity : > 90% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 1xqs. :

Gene Name : HSPA6 heat shock 70kDa protein 6 (HSP70B") [ Homo sapiens ]
Synonyms : HSPA6; heat shock 70kDa protein 6 (HSP70B"); heat shock 70kD protein 6 (HSP70B"); Heat shock 70 kDa protein 6; HSP70B"; Heat shock 70 kDa protein B"
Gene ID : 3310
mRNA Refseq : NM_002155
Protein Refseq : NP_002146
MIM : 140555
UniProt ID : P17066
Chromosome Location : 1q23
Pathway : Antigen processing and presentation; Endocytosis; MAPK signaling pathway
Function : ATP binding; nucleotide binding

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