| Cat. No. : |
GH-181O |
| Description : |
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. |
| Source : |
E. coli. |
| Amino Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala Less than 4% as determined by gel filtration chromatography. |
| Physical Appearance : |
Lyophylized white powder. |
| Purity : |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Formulation : |
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045 mM NaHCO3 previously adjusted pH 9. |
| Solubility : |
It is recommended to reconstitute the lyophilized oGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar. |
| Endotoxin : |
Less than 0.1 ng/µg (IEU/µg) of oplGH (22 K). |
| Protein Content : |
GH.Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.684 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences. 2. Analysis by RP-HPLC, using a calibrated solution of GH-22K-pl as a Reference Standard. |
| Dimers And Aggregates : |
Less than 4% as determined by gel filtration chromatography. |
| Biological Activity : |
oplGH is fully biologically active when compared to World Health Organization (WHO) reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD). |
| Stability : |
Lyophilized oplGH although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution and filter sterilization oGH can be stored at 4℃, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
