Recombinant Human Topoisomerase (DNA) I

• Cat.No.: TOP1-519H
• Product Overview: Recombinant Human Topoisomerase (DNA) I protein (765 amino acids) was expressed inBaculovirussystem.

Specification

• Cat. No.: TOP1-519H
• Description: Human DNA topoisomerase I (topo I) is a monomeric protein of 765 anino acids encoded by a single-copy gene. Based on its function, DNA topoisomerase I has been subdivided into four distinct domains. The N-terminal 214 amino acids of topo I comprise a highly charged N-terminal domain involved in protein-protein interactions with a number of cellular proteins. The highly conserved core domain expanded from amino acids 215 to 636 contains most catalytic residues and retains DNA binding activity. The active C-terminal domain (residues 713-765) is connected to the core domain by a poorly conserved linker domain (residues 636-712).
• Source: Baculovirus.
• Purity: Purity > 95% bySDS-PAGE analysis.
• Endotoxin: 1 unit equals 1 nanogram purified protein. 0.1 – 1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 μl reaction.

Gene Information

• Gene Name: TOP1 topoisomerase (DNA) I [ Homo sapiens ]
• Synonyms: TOP1; topoisomerase (DNA) I; TOPI; DNA topoisomerase1; EC 5.99.1.2; OTTHUMP00000031713; DNA topoisomerase I; topoisomerase I; type I DNA topoisomerase
• Gene ID: 7150
• mRNA Refseq: NM_003286
• Protein Refseq: NP_003277
• MIM: 126420
• UniProt ID: P11387
• Chromosome Location: 20q12-q13.1
• Function: ATP binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; chromatin binding; nucleotide binding; protein binding