Cat. No.:	TOP1-519H
Product Overview:	Recombinant Human Topoisomerase (DNA) I protein (765 amino acids) was expressed inBaculovirussystem.
Description:	Human DNA topoisomerase I (topo I) is a monomeric protein of 765 anino acids encoded by a single-copy gene. Based on its function, DNA topoisomerase I has been subdivided into four distinct domains. The N-terminal 214 amino acids of topo I comprise a highly charged N-terminal domain involved in protein-protein interactions with a number of cellular proteins. The highly conserved core domain expanded from amino acids 215 to 636 contains most catalytic residues and retains DNA binding activity. The active C-terminal domain (residues 713-765) is connected to the core domain by a poorly conserved linker domain (residues 636-712).
Source:	Baculovirus.
Purity:	Purity > 95% bySDS-PAGE analysis.
Endotoxin:	1 unit equals 1 nanogram purified protein. 0.1 – 1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 μl reaction.

Gene Name:	TOP1 topoisomerase (DNA) I [ Homo sapiens ]
Synonyms:	TOP1; topoisomerase (DNA) I; TOPI; DNA topoisomerase1; EC 5.99.1.2; OTTHUMP00000031713; DNA topoisomerase I; topoisomerase I; type I DNA topoisomerase
Gene ID:	7150
mRNA Refseq:	NM_003286
Protein Refseq:	NP_003277
MIM:	126420
UniProt ID:	P11387
Chromosome Location:	20q12-q13.1
Function:	ATP binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; chromatin binding; nucleotide binding; protein binding

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