||Taq DNA Polymerase is a thermostable enzyme of approximately 94kDa purified from the expression product of E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT1. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and also possesses a 5´→3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
||E. colistrain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT1.
||Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions. Specify Your Own Reaction Conditions: Choose either Taq with Mg-free 10× Reaction Buffer and separate 25mM MgCl2or Taq with 10× Reaction Buffer containing 15mM MgCl2. Rely on a Performance-Tested Enzyme: Creative BioMart PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account.
||PCR. 3´ A-tailing of blunt ends, compatible with T-Vectors.
||One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10μg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50μl.
|Quality Control Tests:
||PCR, activity, SDS-PAGE (purity), endonuclease/nickase.
||Taq DNA polymerase in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20 should be stored at -20°C.
|10× Reaction Buffer:
||10× Reaction Buffer with MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP. 10× Reaction Buffer without MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton X-100. Include: 10× Reaction Buffer without MgCl2 and separate 25mM MgCl2Solution.
||To amplify a 1kb DNA template Set up PCR reactions as follows: Taq DNA Polymerase 0.2μl 2.5mM dNTP Mixture 4μl 10× Reaction Buffer w/o MgCl25μl 25mM MgCl23μl 10μM Primers 1μl each Template ~1μl ddH2O 35μl