"Ligase" Related Products

T4 DNA ligase

Cat. No.: CMC12
Description: T4 DNA Ligase is a recombinant enzyme formulated and tested for high activity in blunt- and cohesive-end ligations for cloning and linker addition. The enzyme covalently joins 5"-phosphate and 3"-hydroxyl termini in duplex DNA or RNA. It is free of detectable exo- or endonuclease activities.
Source: Purified from recombinant E. coli strain.
Applications: Cloning of restriction fragments; Joining linkers and adapters to blunt-ended DNA.
Unit Definition (Cohesive End Ligation Unit): One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of lambda DNA (5" DNA termini concentration of 0.12μM, 300μg/ml) in a total reaction volume of 20μl in 30 minutes at 16°C in 1× T4 DNA Ligase Reaction Buffer. One Cohesive End Ligation Unit equals 0.015 Weiss (ATP-PP exchange) units, or one Weiss unit equals 67 Cohesive End Ligation Units.
Concentration: 400,000 units/ml.
Quality Control Tests: Activity, SDS-PAGE (purity), DNase, endonuclease/nickase, RNase.
Storage: T4 DNA ligase in 10mM Tris-HCl, 50mM KCl, 1mM dithiothreitol, 0.1mM EDTA, 0.2 mg/ml BSA, 50% glycerol, pH 7.4 (25℃) should be stored at -20°C.
Heat Inactivation: 65°C for 10 minutes.
1× T4 DNA Ligase Reaction Buffer: 50mM Tris-HCl, 10mM MgCl2, 1mM ATP, 10mM dithiothreitol, 25μg/ml BSA, pH7.5 (25℃)).
Reaction Conditions: 1× T4 DNA Ligase Reaction Buffer, incubate at 16°C. For convenience, ligations may be done at room temperature (20-25°C). For cohesive (sticky) ends, use 1μl of T4 DNA Ligase in a 20μl reaction for 10 minutes. For blunt ends, use 1μl of T4 DNA Ligase in a 20μl reaction for 2 hours.

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.