"Glycosylase" Related Products

Uracil DNA Glycosylase(UNG)

Cat. No.: CMC13
Description: E.coli Uracil DNA Glycosylase (UNG) catalyses the release of free uracil from uracil-containing DNA. UNG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).
Source: An E. coli strain that carries the UNG gene from E. coli.
Applications: Glycosylase mediated single nucleotide polymorphism detection (GMPD); Site-directed mutagenesis; As a probe for protein-DNA interaction studies; Rapid and efficient cloning of PCR products; Elimination carry-over contamination in PCR.
Unit Definition: One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA in a total reaction volume of 50 μl in 30 minutes at 37℃ in 1× Uracil DNA Glycosylase Reaction Buffer with 1 unit of uracil DNA Glycosylase and 0.2 μg [3H]-uracil DNA (104-105 cpm/μg).
Quality Control: Activity, SDS-PAGE (purity), 16-Hour Incubation, Exonuclease and Endonuclease Activity.
Storage: UNG in 10 mM Tris-HCl (pH 7.4 at 25°C), 50 mM KCl, 1 mM Dithiothreitol, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol should be stored at -20℃.
10× UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25°C), 10mM Dithiothreitol, 10mM EDTA.
Reaction Conditions: 1× UNG Reaction Buffer, incubate at 37℃.
Inhibition and Inactivation: Inactivated by heating at 95℃ for 10min. Enzyme activity is partially restored at temperatures lower than 55°C.
Usage notes: UNG is active over a broad pH range with an optimum at pH 8.0, does not require divalent cation, and is inhibited by high ionic strength (> 200 mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.