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Recombinant E. coli ung Protein

Cat.No. : CMC13
Product Overview : Recombinant ung protein was expressed in an E. coli strain that carries the UDG gene from E. coli.
  • Specification
  • Gene Information
  • Related Products
Description : UDG and Ndk form a complex (Goswami, 2006). [More information is available at EcoGene: EG11058]. Uracil can be generated in DNA as a result of misincorporation by DNA polymerase, spontaneous deamination of cytosine, and deamination of cytosine induced by bisulfite or nitrous acid.
Source : E. coli
Species : E. coli
Form : Reagents Supplied
The following reagents are supplied with this product:
10X UDG Reaction Buffer
Unit Definition : One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 µl reaction containing 0.2 µg DNA (10^4-10^5 cpm/µg) in 30 minutes at 37 centigrade.
Applications : Treatment of 0.1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37 centigrade renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95 centigrade for 10 minutes. Since UDG remains partially active following heat treatment at 95 centigrade, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform.
Usage : Reaction Conditions
1X UDG Reaction Buffer
Incubate at 37 centigrade

1X UDG Reaction Buffer
20 mM Tris-HCl
1 mM DTT
1 mM EDTA
(pH 8 @ 25 centigrade)

Heat Inactivation
No

Unit Assay Conditions
1X UDG Reaction Buffer, 1 unit of uracil DNA Glycosylase, 0.2 µg 3H-uracil DNA (10^4-10^5 cpm/μg) for 30 minutes at 37 centigrade in a total reaction volume of 50 µl.
Notes : UDG is active over a broad pH range with an optimum at pH 8.0, does not require divalent cation, and is inhibited by high ionic strength (> 200 mM).
Storage : At -20 centigrade.
Concentration : 5,000 units/mL
Storage Buffer : 10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
0.1 mg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
Gene Name : ung
Official Symbol : ung uracil-DNA-glycosylase [ Escherichia coli str. K-12 substr. MG1655 ]
Synonyms : ung; uracil-DNA-glycosylase; ECK2578; JW2564; uracil-DNA-glycosylase; EC 3.2.2.-
Gene ID : 947067
Protein Refseq : NP_417075
UniProt ID : P12295

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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02/12/2022

    AQP12B protein has been extensively studied and characterized, allowing researchers to confidently integrate it into their experimental workflows.

    11/05/2018

      The manufacturer's commitment to providing excellent technical support is commendable, as their dedicated team possesses the expertise to solve any issue or query that may arise during my experiments.

      09/05/2018

        The AQP2 protein offered by the manufacturer is of exceptional quality and is expected to perfectly meet my experimental needs.

        04/03/2018

          Their knowledgeable team is readily available to address any questions or concerns, guaranteeing a smooth experimental process.

          02/16/2018

            Its unique properties and functions make it an essential component in investigating various biological processes and molecular mechanisms.

            04/04/2017

              Its purity and integrity are of the highest standard, ensuring reliable and reproducible results in my research.

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