||Recombinant Human RIPK2, N-terminal fragment, amino acids M1-E299, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, was expressed inSf9insect cells.MW = 63,815 Da.
||This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-κB and inducer of apoptosis in response to various stimuli.
||Baculovirus infected Sf9 cells.
||RIPK2 was confirmed as RIPK2 by mass spectroscopy LC-ESI-MS/MS.
||50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.206 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!