||Recombinant Human CK2-alpha2 Amino acids M1-R350, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed in Sf9 cells. MW = 71,109 Da.
||Casein kinase II subunit alpha" is an enzyme that in humans is encoded by the CSNK2A2 gene.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||CK2-alpha2 was confirmed as CK2-alpha2 by specific Western Blotting using anti CK2-alpha2 antibody.
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.715 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!