"DTNB-Thiols" Related Products

DTNB-Thiols Assay Kit

Cat.No.: Kit-0311
Product Overview: DTNB-Thiols Assay Kit is used for the quantitiation of Sulfhydryls (DTNB-Thiols).
Description: The DTNB-Thiols assay kit measures sulfhydryl groups with the thiol reagent 5-5dithiobis[2nitrobenzoic acid] (DTNB), which forms the 5-thionitrobenzoic acid and a mixed disulfide. Under conditioins of oxidative stress, free sulfhydryls decrease and disulfides increase. Determination of the free thiol concentration in biological samples reflects the ability to detoxify lipid and other peroxides. Exocell"s DTNB-Thiols assay can be used with a spectrum of biological samples including body fluids, tissue, and cell specimens.
Usage: For research use only (RUO)
Storage: Store this kit at 4°C. Reconstituted Ellman"s Reagent and DTNB Thiols are not stable and should be discarded after 1 day.
Kit Components: 96 well microtiter plate. 96 (8x12) wellsDTNB (Ellman"s Reagent) 1 vialEllman"s Reagent Diluent 1 vialDTNB Thiols (L-Cysteine Standard 10.0 mg vial) 1 vialDTNB Thiols Assay Diluent 1 via
Detection method: Colorimetric
Compatible Sample Types: Body fluids, Cell specimens and Tissue

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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