Active GMP Recombinant Human EPCAM protein

• Cat.No.: EPCAM-186HG
• Product Overview: GMP Recombinant Human EPCAM protein(NP_002345.2)(241 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Source: HEK293
• Species: Human
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: The determination is based on the ability of the immobilized protein to support the adhesion of the L cell-mouse fibroblast cell line. When 5×104 cells/well were added to Recombinant Human EpCAM and human fibronectin coated plates (0.5ug/ml), cell adhesion was enhanced in a dose-dependent manner. The ED50 of this effect is 1-2.4ug/ml.
• Molecular Mass: Human EpCAM Protein contains 241aa. It has a predicted MW of 29.1 kDa.
• Protein length: DNA sequence encoding Human EpCAM(NCBI Reference Sequence: NP_002345.2) was expressed with His tag at the C-terminal.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPCAM epithelial cell adhesion molecule [ Homo sapiens ]
• Official Symbol: EPCAM
• Synonyms: EPCAM; TACSTD1; TROP1; CD326; DIAR5; EGP2; EGP314; EGP40; ESA; GA733-2; HNPCC8; HNPCC-8; KS1/4; KSA; M4S1; MIC18; MK1
• Gene ID: 4072
• mRNA Refseq: NM_002354
• Protein Refseq: NP_002345
• MIM: 185535
• UniProt ID: P16422