|Product Overview:||Recombinant Human FGF2, Pro143-Ser288, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.|
|Bio-activity:||Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.
The specific activity of Recombinant Human FGF basic GMP is approximately 800 IU/μg, which is calibrated against human FGF basic WHO International Standard (NIBSC code: 90/712).
|Molecular Mass:||Predicted Molecular Mass: 16.5 kDa
SDS-PAGE: 17 kDa, reducing conditions
|N-terminal Sequence Analysis:||Ala-Pro143-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser|
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens ]|
|Synonyms:||FGF2; fibroblast growth factor 2 (basic); FGFB; fibroblast growth factor 2; prostatropin; BFGF; FGF-2; HBGF-2;|