Isopropanol Dehydrogenase Colorimetric Activity Assay Kit

• Cat.No.: Kit-2133
• Product Overview: Isopropanol Dehydrogenase Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the ADH1- NADP in a variety of samples. In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+ . This results in the development of colour in the presence of the developer. This colour is proportional to the ADH1-NADP levels in the sample and can be measured at λ = 450 nm. This assay detects ADH1-NADP activity as low as 0.001 mU in samples.

Specification

• Size: 100 assays
• Applications: Measurement of Isopropanol Dehydrogenase (ADH1-NADP) activity in various cells.Measurement of NADP-dependent alcohol dehydrogenases" activity with broad substrate specificity in various tissues/cells.
• Storage: Store the kit at -20°C, protected from light. Briefly centrifuge vials prior to opening. Read the entire protocol before performing the assay.ADH1-NADP Assay Buffer: Warm to room temperature before use. Store at either 4ºC or -20ºC.ADH1-NADP Developer: Reconstitute the Developer with 0.9 ml of ddH2O. Pipette up and down several times to completely dissolve the pellet (Do not vortex). Aliquot and store at –20°C. Keep on ice while in use. Use within two months.ADH1-NADP Positive Control: Dilute the Positive Control with 100 µl Assay Buffer and mix thoroughly. Aliquot and store directly at -20°C. Keep on ice while in use. Use within two months.NADPH Standard: Reconstitute NADPH Standard with 200 µl pure DMSO to generate 1 mM NADPH Standard solution. Aliquot and store at –20°C. Keep on ice while in use. Use within two months.

Reviews

04/06/2018

Demonstrates exceptional reproducibility in protein crystallization experiments.

12/26/2016

Displays remarkable catalytic efficiency in enzyme kinetics assays.

Q&As

Can ADH1-NADP metabolize other substrates apart from alcohol, and what are the implications for its broader metabolic role?

ADH1-NADP may have the capability to metabolize other substrates besides alcohol, suggesting its involvement in broader metabolic pathways and potential metabolic interactions.

What is the evolutionary conservation of ADH1-NADP across different species, and are there any species-specific differences in its expression or enzymatic properties?

ADH1-NADP shows evolutionary conservation across different species, but there may be species-specific differences in its expression levels or enzymatic properties.

Are there any known genetic variations or polymorphisms in the ADH1-NADP gene associated with altered alcohol metabolism or susceptibility to alcohol-related disorders?

Genetic variations or polymorphisms in the ADH1-NADP gene may influence alcohol metabolism or susceptibility to alcohol-related disorders, emphasizing the impact of genetic factors on its function.

What is the specific role of ADH1-NADP in alcohol metabolism, and how does it differ from other ADH isoforms in terms of its enzymatic properties and substrate specificity?

ADH1-NADP plays a specific role in alcohol metabolism, exhibiting unique enzymatic properties and substrate specificity compared to other ADH isoforms.

Can ADH1-NADP contribute to the detoxification of other substances or play a role in drug metabolism, and if so, what are the implications for therapeutic applications?

ADH1-NADP might contribute to the detoxification of other substances or play a role in drug metabolism, highlighting its potential therapeutic applications beyond alcohol metabolism.

How does ADH1-NADP respond to pharmacological agents or environmental factors, and can its activity be modulated by specific compounds or interventions?

ADH1-NADP may respond to pharmacological agents or environmental factors, and its activity can be modulated by specific compounds or interventions, providing potential therapeutic opportunities.

How does the expression of ADH1-NADP vary across different tissues and cell types, and what are the underlying regulatory mechanisms governing its expression?

The expression of ADH1-NADP shows tissue-specific variations, and its regulation involves specific mechanisms such as transcriptional control and post-translational modifications.

What are the structural features of ADH1-NADP, and how do they contribute to its catalytic activity and binding affinity for NADP?

The structural features of ADH1-NADP, including its active site and binding domains, contribute to its catalytic activity and its affinity for NADP.

What are the functional consequences of ADH1-NADP deficiency or knockout in experimental models, and how do they impact alcohol metabolism and related phenotypes?

Experimental models with ADH1-NADP deficiency or knockout exhibit altered alcohol metabolism and related phenotypes, indicating the significance of ADH1-NADP in these processes.

Are there any interacting proteins or cofactors that modulate the activity or localization of ADH1-NADP, and how do they influence its enzymatic function?

Interacting proteins or cofactors may modulate the activity or localization of ADH1-NADP, potentially influencing its enzymatic function and cellular processes.