LSD1 Demethylase Activity/Inhibition Colorimetric Assay Kit
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|Product Overview :||LSD1 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening LSD1 demethylase inhibitors.|
|Applications :||The LSD1 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is suitable for measuring LSD1 activity/inhibition using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including cultured cells and fresh tissues. Nuclear extracts can be prepared by using your own successful method. Nuclear extracts can be used immediately or stored at -80°C for future use. Purified enzymes can be active LSD1 from recombinant proteins or isolated from cell/tissues.|
|Storage :||Upon receipt: (1) Store LD3, LD4, and LD6 at –20°C away from light; (2) Store LD1, LD5, LD7, LD8, and 8-Well Assay Strips at 4°C away from light; (3) Store remaining components (LD2, LD9, and Adhesive Covering Film) at room temperature away from light.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: (1) Check if LD1 (10X Wash Buffer) contains salt precipitates before use. If so, warm (at room temperature or 37°C) and shake the buffer until the salts are re-dissolved; and (2) check if a blue color is present in LD8 (Developer Solution), which would indicate contamination of the solution and should not be used. To avoid contamination, transfer the amount of LD8 required into a secondary container (tube or vial) before adding LD8 into the assay wells.|
◆ Assay kits
|Kit-0524||LSD1 Demethylase Activity/Inhibition Fluorometric Assay Kit||+Inquiry|