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LSD1 Demethylase Activity/Inhibition Colorimetric Assay Kit

Cat.No. : Kit-0523
Product Overview : LSD1 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening LSD1 demethylase inhibitors.
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Applications : The LSD1 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is suitable for measuring LSD1 activity/inhibition using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including cultured cells and fresh tissues. Nuclear extracts can be prepared by using your own successful method. Nuclear extracts can be used immediately or stored at -80°C for future use. Purified enzymes can be active LSD1 from recombinant proteins or isolated from cell/tissues.
Storage : Upon receipt: (1) Store LD3, LD4, and LD6 at –20°C away from light; (2) Store LD1, LD5, LD7, LD8, and 8-Well Assay Strips at 4°C away from light; (3) Store remaining components (LD2, LD9, and Adhesive Covering Film) at room temperature away from light.All components of the kit are stable for 6 months from the date of shipment, when stored properly.Note: (1) Check if LD1 (10X Wash Buffer) contains salt precipitates before use. If so, warm (at room temperature or 37°C) and shake the buffer until the salts are re-dissolved; and (2) check if a blue color is present in LD8 (Developer Solution), which would indicate contamination of the solution and should not be used. To avoid contamination, transfer the amount of LD8 required into a secondary container (tube or vial) before adding LD8 into the assay wells.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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