m7GTP Agarose

• Cat.No.: m7GTP-001A
• Product Overview: Immobilized γ-Aminophenyl-7-Methyl-guanosine 5'-triphosphate (m7GTP) is suitable for purification of eukaryotic mRNA cap-binding proteins.

Specification

• Size: 1 mL, 5 mL
• Sterilization: Not autoclavable
• Matrix: Highly cross-linked agarose
• Average particle size: 45 - 165 μm
• Ligand: m7GTP
• Ligand concentration: 5-7 umol m7GTP/ml gel
• Recommended flow rate: 11.5-150 cm/h
• Chemical stability: Stable to all solutions commonly used in gel filtration including 8 M urea and 6 M guanidine hydrochloride. Not stable in organic solvents.
• pH working range: Short term: 4-9 Long term: 7.5
• pH stability: 4-9
• Maximum pressure: 0.25 bar(3.6 psi)
• pH CIP range: 4-9
• Shelf life: 24 months
• Publications: Estrogen receptor α promotes protein synthesis by fine-tuning the expression of the eukaryotic translation initiation factor 3 subunit f (eIF3f) (2018) A novel role of the vaccinia virus mRNA capping enzyme in determining virus host range (2018) Hematopoietic Stem and Progenitor Cells Exhibit Stage-Specific Translational Programs via mTOR- and CDK1-Dependent Mechanisms (2020) YWHAE/14-3-3ε expression impacts the protein load, contributing to proteasome inhibitor sensitivity in multiple myeloma (2020) RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site (2021) eIF4E assembly into C. elegans germ granules is essential for its repressive function (2025)
• Storage: Store at 4°C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Storage Buffer: 20 % Ethanol
• Applications: Suitable for purification of eukaryotic mRNA cap-binding proteins.
• Shipping: Shipped at 4 centigrade.
• Notes: For general laboratory use.
• Publications:
  • Estrogen receptor α promotes protein synthesis by fine-tuning the expression of the eukaryotic translation initiation factor 3 subunit f (eIF3f) (2018)
  • A novel role of the vaccinia virus mRNA capping enzyme in determining virus host range (2018)
  • Hematopoietic Stem and Progenitor Cells Exhibit Stage-Specific Translational Programs via mTOR- and CDK1-Dependent Mechanisms (2020)
  • YWHAE/14-3-3ε expression impacts the protein load, contributing to proteasome inhibitor sensitivity in multiple myeloma (2020)
  • RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site (2021)
  • eIF4E assembly into C. elegans germ granules is essential for its repressive function (2025)