| Species : |
Pig |
| Source : |
Blood |
| Description : |
Porcine SOD is a copper-zinc superoxide dismutase with a molecular weight of 32,000 Da. It forms a non-covalent homodimer from four subunits. Each subunit, composed of 152 amino acids and with a molecular weight of 16,000 Da, contains one disulfide bond and one free thiol group. |
| Molecular Mass : |
32 kDa |
| Extinction Coefficient : |
1E^mM = 10.3 (258nM)
No significant peak at 280 nm due to the absence of tryptophan. |
| Optimal pH : |
7.8 |
| Optimal Temperature : |
25 centigrade |
| Isoelectric Point : |
4.95 |
| Inhibitors : |
Cyanide, OH-(competitive), hydrogen peroxide. |
| Unit Definition : |
At a constant temperature of 25 centigrade, one unit of enzyme activity is defined as the amount of enzyme required to inhibit the autoxidation rate of pyrogallol by 50% per minute in one milliliter of reaction mixture. |
| Stability : |
Lyophilized Powder: Stable for over three years at -20 centigrade. Stable for one year at 4 centigrade. Stable for six months at 25 centigrade.
1 mg/mL Solution in 0.1M Potassium Phosphate Buffer (pH 7.5): Stable for one hour at 60 centigrade. Stable for six hours at 25 centigrade. Stable for two days at 4 centigrade. Stable for over one year at -20 centigrade. |
| Reconstitution : |
SOD lyophilized powder is light blue-green. Dissolve it in 0.1 M potassium phosphate buffer (pH 7.5) to make a 1 mg/mL solution and aliquot for storage. |
