Native Pig Superoxide Dismutase, GMP grade

Species :	Pig
Source :	Blood
Description :	Porcine SOD is a copper-zinc superoxide dismutase with a molecular weight of 32,000 Da. It forms a non-covalent homodimer from four subunits. Each subunit, composed of 152 amino acids and with a molecular weight of 16,000 Da, contains one disulfide bond and one free thiol group.
Molecular Mass :	32 kDa
Extinction Coefficient :	1E^mM = 10.3 (258nM) No significant peak at 280 nm due to the absence of tryptophan.
Optimal pH :	7.8
Optimal Temperature :	25 centigrade
Isoelectric Point :	4.95
Inhibitors :	Cyanide, OH-(competitive), hydrogen peroxide.
Unit Definition :	At a constant temperature of 25 centigrade, one unit of enzyme activity is defined as the amount of enzyme required to inhibit the autoxidation rate of pyrogallol by 50% per minute in one milliliter of reaction mixture.
Stability :	Lyophilized Powder: Stable for over three years at -20 centigrade. Stable for one year at 4 centigrade. Stable for six months at 25 centigrade. 1 mg/mL Solution in 0.1M Potassium Phosphate Buffer (pH 7.5): Stable for one hour at 60 centigrade. Stable for six hours at 25 centigrade. Stable for two days at 4 centigrade. Stable for over one year at -20 centigrade.
Reconstitution :	SOD lyophilized powder is light blue-green. Dissolve it in 0.1 M potassium phosphate buffer (pH 7.5) to make a 1 mg/mL solution and aliquot for storage.