|Product Overview :||Recombinant Cynomolgus IL5 Protein (Met1-Ser134) was produced by Baculovirus-Insect Cells expression system. This protein was expressed with a polyhistidine tag at the C-terminus.|
|Description :||Interleukin 5 (IL-5) is a member of the interleukin family with length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins / signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered as a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues, and can modulate eosinophil behavior at every stage from maturation to survival.|
|Source :||Baculovirus-Insect Cells|
|Predicted N Terminal :||Ile 20|
|Form :||Lyophilized from sterile 50mM Tris, pH 8.0,150mM NaCl,10% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.|
|Bio-activity :||Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
The EC50 for this effect is typically 0.5-2ng/ml.
|Molecular Mass :||The recombinant IL5 consists of 126 amino acids and predicts a molecular mass of 14.6 kDa.|
|Protein length :||Met1-Ser134|
|Endotoxin :||< 1.0 EU per μg protein as determined by the LAL method.|
|Purity :||> 95 % as determined by SDS-PAGE.|
|Stability :||Samples are stable for up to twelve months from date of receipt at -70 centigrade.|
|Storage :||Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|Shipping :||In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.