Recombinant E.coli ppa, His-tagged

• Cat.No.: ppa-3594E
• Product Overview: Recombinant E.coli ppa protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-176aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
• Molecular Weight: 21.9kDa (196aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Oxidative phosphorylation, organism-specific biosystem; Oxidative phosphorylation, conserved biosystem

Gene Information

• Gene Name: ppa inorganic pyrophosphatase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: ppa
• Synonyms: ppa; inorganic pyrophosphatase; EC 3.6.1.1
• Gene ID: 948748
• Protein Refseq: NP_418647