Active Recombinant Feline FCGRT & B2M Heterodimer Protein, His & Avi-tagged, Biotinylated

• Cat.No.: FCGRT & B2M-379F
• Product Overview: Recombinant Feline FCGRT & B2M Heterodimer Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Val 21 - Met 118 (B2M) (Accession # W5VRL5-1 (FCGRT) & Q5MGS7-1 (B2M)). Predicted N-terminus: Ala 24 (FCGRT) & Val 21 (B2M). The subunit FCGRT carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 34.0 kDa (FCGRT) and 11.5 kDa (B2M) . The protein migrates as 36-40 kDa (FCGRT) and 18-20 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Source: HEK293
• Species: Feline
• Tag: His & Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Ipilumimab at 5 μg/mL (100 μL/well) can bind Recombinant Feline FCGRT & B2M Heterodimer Protein, His & Avi-tagged, Biotinylated with a linear range of 0.02-0.156 μg/mL (QC tested).
• Molecular Mass: 34.0 kDa (FCGRT) and 11.5 kDa (B2M)
• Protein length: Ala 24 - Ser 297 (FCGRT) & Val 21 - Met 118 (B2M)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.