Active Recombinant Human FCGRT & B2M protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: FCGRT & B2M-1050H
• Product Overview: Biotinylated Active Recombinant Human FCGRT & B2M protein(P55899(FCGRT) & P61769((B2M))(Ala24-Ser297(FCGRT)&Ile21-Met119(B2M)) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 24-297 aa(FCGRT) & 21-119 aa(B2M)
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Biotinylated Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 0.557 uM asdetermined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ]
• Official Symbol: FCGRT
• Synonyms: FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; alpha chain; FCRN; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; alpha-chain
• Gene ID: 2217
• mRNA Refseq: NM_001136019
• Protein Refseq: NP_001129491
• MIM: 601437
• UniProt ID: P55899
• Gene Name 2: B2M beta-2-microglobulin [ Homo sapiens ]
• Official Symbol 2: B2M
• Synonyms 2: B2M; beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• Gene ID 2: 567
• mRNA Refseq 2: NM_004048
• Protein Refseq 2: NP_004039
• MIM 2: 109700
• UniProt ID 2: P61769