Recombinant Human FCGRT & B2M protein(Met1-Ser297 & Met1-Met119), His-tagged

• Cat.No.: FCGRT & B2M-1050H
• Product Overview: Recombinant Human FCGRT (P55899-1)(Met1-Ser297) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human B2M (P61770-1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser297 & Met1-Met119
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human IgG1 in a functional ELISA. 2.Loaded Human FcRn recombinant protein on His1K Biosensor, can bind IgG1 with an affinity constant of 0.11 μM as determined in a BLI assay (ForteBio Octet Red384). 3.Loaded Human FcRn recombinant protein on His1k Biosensor, can bind IgG4 Fc with an affinity constant of 0.08 μM as determined in a BLI assay (ForteBio Octet Red384). 4. Captured Human FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.11 μM as determined in an SPR assay (Biacore T200). 5. Captured Human FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.23 μM as determined in an SPR assay (Biacore T200).
• Molecular Mass: The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. The apparent molecular mass of human FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ]
• Official Symbol: FCGRT
• Synonyms: FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; alpha chain; FCRN; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; alpha-chain
• Gene ID: 2217
• mRNA Refseq: NM_001136019
• Protein Refseq: NP_001129491
• MIM: 601437
• UniProt ID: P55899