Recombinant Human FCGRT & B2M protein(Met1-Ser297 & Met1-Met119), His-tagged
| Cat.No. : | FCGRT & B2M-1050H |
| Product Overview : | Recombinant Human FCGRT (P55899-1)(Met1-Ser297) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human B2M (P61770-1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified in HEK293. |
- Specification
- Gene Information
- Related Products
| Source : | HEK293 |
| Species : | Human |
| Tag : | C-His |
| Protein length : | Met1-Ser297 & Met1-Met119 |
| Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : | 1. Measured by its ability to bind human IgG1 in a functional ELISA. 2.Loaded Human FcRn recombinant protein on His1K Biosensor, can bind IgG1 with an affinity constant of 0.11 μM as determined in a BLI assay (ForteBio Octet Red384). 3.Loaded Human FcRn recombinant protein on His1k Biosensor, can bind IgG4 Fc with an affinity constant of 0.08 μM as determined in a BLI assay (ForteBio Octet Red384). 4. Captured Human FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.11 μM as determined in an SPR assay (Biacore T200). 5. Captured Human FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.23 μM as determined in an SPR assay (Biacore T200). |
| Molecular Mass : | The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. The apparent molecular mass of human FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions. |
| Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
| Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name : | FCGRT Fc fragment of IgG, receptor, transporter, alpha [ Homo sapiens ] |
| Official Symbol : | FCGRT |
| Synonyms : | FCGRT; Fc fragment of IgG, receptor, transporter, alpha; IgG receptor FcRn large subunit p51; alpha chain; FCRN; FcRn alpha chain; neonatal Fc receptor; neonatal Fc-receptor for Ig; IgG Fc fragment receptor transporter alpha chain; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; alpha-chain; |
| Gene ID : | 2217 |
| mRNA Refseq : | NM_001136019 |
| Protein Refseq : | NP_001129491 |
| MIM : | 601437 |
| UniProt ID : | P55899 |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket
