Active Recombinant Full Length Human MAPK3 Protein, GST-tagged

• Cat.No.: MAPK3-2068HF
• Product Overview: Full-length human Erk1 [1-379(end) amino acids of accession number NP_002737.1] was expressed as N- terminal GST-fusion protein (70 kDa) using E.coli expression system. GST-Erk1 was purified by using glutathione sepharose chromatography and activated with His-tagged MAP2K1. Activated GST-Erk1 was purified using glutathione sepharose chromatography.

Specification

• Source: E. coli
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-379
• Purity: 90%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase 3
• Official Symbol: MAPK3
• Synonyms: MAPK3;ERK-1;ERK1;ERT2;HS44KDAP;HUMKER1A;P44ERK1;P44MAPK;PRKM3;p44-ERK1;p44-MAPK;p44erk1;p44mapk
• Protein Refseq: NP_002737.1
• UniProt ID: P27361