Recombinant Full Length Human NOP2/Sun domain family, member 2 Protein, His&StrepII tagged

• Cat.No.: NSUN2-091H
• Product Overview: Recombinant human NSUN2 protein (Q08J23) (Met 1-Arg 767) with a Strep II tag at the N-terminus and a His tag at the C-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His&Strep II
• Protein Length: 1-767aa
• Description: RNA cytosine C5-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C5-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA (Leu) (CAA) precursors, and at positions 48, 49 and 50 of tRNA (Gly) (GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C5-methylation of mitochondrial tRNAs. Catalyzes cytosine C5-methylation of mRNAs, leading to stabilize them and prevent mRNA decay: mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts. Cytosine C5-methylation of mRNAs also regulates mRNA export: methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling. Also mediates cytosine C5-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs. Cytosine C5-methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.
• Tag: N-StrepII and C-His
• Molecular Mass: 89.5 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: NSUN2 NOP2/Sun domain family, member 2 [ Homo sapiens (human) ]
• Official Symbol: NSUN2
• Synonyms: NSUN2; NOP2/Sun domain family, member 2; NOL1/NOP2/Sun domain family 2, NOL1/NOP2/Sun domain family, member 2; tRNA (cytosine(34)-C(5))-methyltransferase; FLJ20303; Misu; Myc induced SUN domain containing protein; TRM4; tRNA methyltransferase 4 homolog (S. cerevisiae); hTrm4; NOL1/NOP2/Sun domain family 2; tRNA methyltransferase 4 homolog; substrate of AIM1/Aurora kinase B; 5-methycytoisine methyltransferase; NOL1/NOP2/Sun domain family member 2; NOL1/NOP2/Sun domain family, member 2; Myc-induced SUN-domain-containing protein; myc-induced SUN domain-containing protein; tRNA (cytosine-5-)-methyltransferase NSUN2; MISU; SAKI
• Gene ID: 54888
• mRNA Refseq: NM_001193455
• Protein Refseq: NP_001180384
• MIM: 610916
• UniProt ID: Q08J23