Recombinant Full Length Human NSUN2 Protein, C-Flag-tagged

• Cat.No.: NSUN2-1126HFL
• Product Overview: Recombinant Full Length Human NSUN2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 86.3 kDa
• AA Sequence: MGRRSRGRRLQQQQRPEDAEDGAEG GGKRGEAGWEGGYPEIVKENKLFEH YYQELKIVPEGEWGQFMDAL REPLPATLRITGYKSHAKEILHCLK NKYFKELEDLEVDGQKVEVPQPLSW YPEELAWHTNLSRKILRKSP HLEKFHQFLVSETESGNISRQEAVS MIPPLLLNVRPHHKILDMCAAPGSK TTQLIEMLHADMNVPFPEGF VIANDVDNKRCYLLVHQAKRLSSPC IMVVNHDASSIPRLQIDVDGRKEIL FYDRILCDVPCSGDGTMRKN IDVWKKWTTLNSLQLHGLQLRIATR GAEQLAEGGRMVYSTCSLNPIEDEA VIASLLEKSEGALELADVSN ELPGLKWMPGITQWKVMTKDGQWFT DWDAVPHSRHTQIRPTMFPPKDPEK LQAMHLERCLRILPHHQNTG GFFVAVLVKKSSMPWNKRQPKLQGK SAETRESTQLSPADLTEGKPTDPSK LESPSFTGTGDTEIAHATED LENNGSKKDGVCGPPPSKKMKLFGF KEDPFVFIPEDDPLFPPIEKFYALD PSFPRMNLLTRTTEGKKRQL YMVSKELRNVLLNNSEKMKVINTGI KVWCRNNSGEEFDCAFRLAQEGIYT LYPFINSRIITVSMEDVKIL LTQENPFFRKLSSETYSQAKDLAKG SIVLKYEPDSANPDALQCPIVLCGW RGKASIRTFVPKNERLHYLR MMGLEVLGEKKKEGVILTNESAAST GQPDNDVTEGQRAGEPNSPDAEEAN SPDVTAGCDPAGVHPPRTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: NSUN2 NOP2/Sun RNA methyltransferase 2 [ Homo sapiens (human) ]
• Official Symbol: NSUN2
• Synonyms: MISU; MRT5; SAKI; TRM4
• Gene ID: 54888
• mRNA Refseq: NM_017755.6
• Protein Refseq: NP_060225.4
• MIM: 610916
• UniProt ID: Q08J23