Active Recombinant Full Length Human PRMT1 Protein, C-Flag-tagged

• Cat.No.: PRMT1-309HFL
• Product Overview: Recombinant Full Length Human PRMT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: In vitro methylation assay (enzyme)
• Molecular Mass: 42.3 kDa
• AA Sequence: MAAAEAANCIMENFVATLANGMSLQ PPLEEVSCGQAESSEKPNAEDMTSK DYYFDSYAHFGIHEEMLKDE VRTLTYRNSMFHNRHLFKDKVVLDV GSGTGILCMFAAKAGARKVIGIECS SISDYAVKIVKANKLDHVVT IIKGKVEEVELPVEKVDIIISEWMG YCLFYESMLNTVLYARDKWLAPDGL IFPDRATLYVTAIEDRQYKD YKIHWWENVYGFDMSCIKDVAIKEP LVDVVDPKQLVTNACLIKEVDIYTV KVEDLTFTSPFCLQVKRNDY VHALVAYFNIEFTRCHKRTGFSTSP ESPYTHWKQTVFYMEDYLTVKTGEE IFGTIGMRPNAKNNRDLDFT
  IDLDFKGQLCELSCSTDYRMRTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: PRMT1 protein arginine methyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: PRMT1
• Synonyms: ANM1; HCP1; IR1B4; HRMT1L2
• Gene ID: 3276
• mRNA Refseq: NM_001536.6
• Protein Refseq: NP_001527.3
• MIM: 602950
• UniProt ID: Q99873