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Active Recombinant Full Length Human SMAD2 Protein, C-Flag-tagged

Cat.No. : SMAD2-350HFL
Product Overview : Recombinant Full Length Human SMAD2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.
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  • Gene Information
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Description : The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Source : Mammalian cells
Species : Human
Tag : Flag
Form : 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bio-activity : Co-immunoprecipitation
Molecular Mass : 52.1 kDa
AA Sequence : MSSILPFTPPVVKRLLGWKKSAGGS GGAGGGEQNGQEEKWCEKAVKSLVK KLKKTGRLDELEKAITTQNC NTKCVTIPSTCSEIWGLSTPNTIDQ WDTTGLYSFSEQTRSLDGRLQVSHR KGLPHVIYCRLWRWPDLHSH HELKAIENCEYAFNLKKDEVCVNPY HYQRVETPVLPPVLVPRHTEILTEL PPLDDYTHSIPENTNFPAGI EPQSNYIPETPPPGYISEDGETSDQ QLNQSMDTGSPAELSPTTLSPVNHS LDLQPVTYSEPAFWCSIAYY ELNQRVGETFHASQPSLTVDGFTDP SNSERFCLGLLSNVNRNATVEMTRR HIGRGVRLYYIGGEVFAECL SDSAIFVQSPNCNQRYGWHPATVCK IPPGCNLKIFNNQEFAALLAQSVNQ GFEAVYQLTRMCTIRMSFVK
GWGAEYRRQTVTSTPCWIELHLNGP LQWLDKVLTQMGSPSVRCSSMSTRT RPLEQKLISEEDLAANDILDYKDDD DKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining.
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade.
Concentration : >50 ug/mL as determined by microplate BCA method.
Preparation : Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Protein Families : Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
Protein Pathways : Adherens junction, Cell cycle, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway
Gene Name : SMAD2 SMAD family member 2 [ Homo sapiens (human) ]
Official Symbol : SMAD2
Synonyms : JV18; LDS6; CHTD8; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Gene ID : 4087
mRNA Refseq : NM_005901.6
Protein Refseq : NP_005892.1
MIM : 601366
UniProt ID : Q15796

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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