Recombinant HIV-1 GP120, His-tagged

• Cat.No.: GP120-5227H
• Product Overview: Recombinant HIV-1 GP120 Protein (HIV-1 /Clade B/C (CN54)) (rCN54 /GP120) derived from the env. gene of HIV-1 strain CN54 gp160 (Thr27-Arg498) and glycosylated with N-linked sugars and expressed in HEK293 cells

Specification

• Species: HIV
• Source: HEK293
• Tag: His
• Description: Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses.
• Predicted N Terminal: Thr27
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization
• Molecular Mass: 53.8 kDa. DTT-reduced protein migrates as 80-110 kDa protein due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the or rCN54 /GP120 by the LAL method.
• Purity: >97% purity as determined by SDS-PAGE of reduced rCN54 /GP120.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).