Active Recombinant Human APOE4 protein(C130R), His-tagged

• Cat.No.: APOE4-2872H
• Product Overview: Recombinant Human APOE protein(P02649-1 (C130R))(Lys 19-His 317),fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 19-317 aa (C130R)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 5 mM CHAPS, 1 mM PMSF, pH7.4 with trehalose as protectant.
• Bio-activity: Loaded Human TREM2, Mouse IgG2a Fc Tag on Protein A Biosensor, can bind Human APOE4, His Tag with an affinity constant of 37.6 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: The protein has a calculated MW of 36.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: APOE apolipoprotein E [ Homo sapiens ]
• Official Symbol: APOE
• Synonyms: APOE; apolipoprotein E; AD2; LPG; LDLCQ5; MGC1571
• Gene ID: 348
• mRNA Refseq: NM_000041
• Protein Refseq: NP_000032
• UniProt ID: P02649