Active Recombinant Human ARMET protein

• Cat.No.: ARMET-837H
• Product Overview: Human ARMET (P55145) partial recombinant protein expressed in E. coli.

Specification

• Description: The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]
• Source: E. coli
• Species: Human
• Form: Lyophilized
• Bio-activity: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 15-25 ug/ml.
• Molecular Mass: 18.1 kDa
• AA Sequence: LRPGDCEVCISYLGRFYQDLKDRDV TFSPATIENELIKFCREARGKENRL CYYIGATDDAATKIINEVSKPLAHH IPVEKICEKLKKKDSQICELKYDKQ IDLSTVDLKKLRVKELKKILDDWGE TCKGCAEKSDYIRKINELMPKYAPK AASARTDL
• Endotoxin: <0.1 ng/ug (<1 EU/ug)
• Purity: 98%
• Applications: Functional Study; SDS-PAGE
• Usage: SDS-PAGE
  Activity assay
  The optimal working dilution should be determined by the end user.
• Storage: Store at -20 centigrade.Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex.For extended storage, it is recommended to be further diluted in buffer containing carrier protein (e.g. 0.1% BSA) and stored in aliquots at -20 centigrade to -80 centigrade.
• Storage Buffer: Lyophilized from 10mM Sodium Phosphate, pH 7.0.

Gene Information

• Gene Name: MANF mesencephalic astrocyte-derived neurotrophic factor [ Homo sapiens ]
• Official Symbol: MANF
• Synonyms: MANF; mesencephalic astrocyte-derived neurotrophic factor; arginine rich, mutated in early stage tumors , ARMET; ARP; arginine-rich, mutated in early stage tumors; ARMET; MGC142148; MGC142150
• Gene ID: 7873
• mRNA Refseq: NM_006010
• Protein Refseq: NP_006001
• MIM: 601916
• UniProt ID: P55145