Recombinant Human CCL4 Protein (69 aa) CCL4-329C

Recombinant Human CCL4 Protein (69 aa)

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Recombinant Human CCL4 Protein (69 aa)

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Cat.No. : CCL4-329C Optional Service: Optional requirements on this protein
Product Overview : Recombinant MIP-1 beta/CCL4 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rhMIP-1 beta/CCL4 has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Description : Macrophage inflammatory protein 1 beta (MIP-1β), also known as Chemokine (C-C motif) ligand 4(CCL4), is a small cytokine belonging to the CC chemokine family. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. MIP-1βis a major HIV-suppressive factor produced by CD8+ T cells. Perforin-low memory CD8+ T cells are the most common T-cells that normally synthesize MIP-1-beta in humans. MIP-1βhas been shown to interact with CCL3. It can signal through the CCR5 receptor.
Source : E. coli
Species : Human
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : The EC50 value of human MIP-1 beta/CCL4 on Ca^2+ mobilization assay in CHO-K1/Gα15/hCCR5 cells (human Gα15 and human CCR5 stably expressed in CHO-K1 cells) is less than 100 ng/mL.
Molecular Mass : 7.6 kDa, observed by reducing SDS-PAGE.
Protein Length : 69
AA Sequence : APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN
Endotoxin : < 0.2 EU/μg, determined by LAL method.
Purity : > 95% as analyzed by SDS-PAGE and HPLC.
Storage : Lyophilized recombinant human MIP-1 beta/CCL4 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, human MIP-1 beta/CCL4 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
Storage Buffer : Lyophilized after extensive dialysis against PBS.
Reconstitution : Reconstituted in ddH2O or PBS at 100 μg/mL.
Gene Name : CCL4 chemokine (C-C motif) ligand 4 [ Homo sapiens ]
Official Symbol : CCL4
Synonyms : CCL4; chemokine (C-C motif) ligand 4; LAG1, SCYA4, small inducible cytokine A4 (homologous to mouse Mip 1b); C-C motif chemokine 4; Act 2; AT744.1; MIP 1 beta; PAT 744; SIS-gamma; MIP-1-beta(1-69); CC chemokine ligand 4; secreted protein G-26; T-cell activation protein 2; small-inducible cytokine A4; lymphocyte-activation gene 1; G-26 T-lymphocyte-secreted protein; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; small inducible cytokine A4 (homologous to mouse Mip-1b); ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; MIP-1-beta; MGC104418; MGC126025; MGC126026;
Gene ID : 6351
mRNA Refseq : NM_002984
Protein Refseq : NP_002975
MIM : 182284
UniProt ID : P13236
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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