Recombinant Human CDK5 protein, GST-tagged

• Cat.No.: CDK5-1H
• Product Overview: Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Description: CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5. CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction.
• Source: Sf9 insect cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mMDTT, O.1 mM PMSF, 25% glycerol.
• Molecular Mass: ~59 kDa and p25 ~49 kDa
• Purity: >95% by densitometry
• Applications: Cancer, Cell Cycle, Ser/Thr Kinases
• Stability: 1yr At -70*C from date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: CDK5 cyclin dependent kinase 5 [ Homo sapiens (human) ]
• Official Symbol: CDK5
• Synonyms: PSSALRE p25; CDK5R1; CDK5P35, CDK5R, NCK5A, p23, p35, p35nck5a
• Gene ID: 1020
• mRNA Refseq: NM_004935
  NM_003885
• Protein Refseq: NP_004926
  NP_003876
• MIM: 123831
• UniProt ID: Q00535