Recombinant Human CDK8/CCNC protein, GST-tagged CDK8-CCNC-314H

Cat.No.:	CDK8-CCNC-314H
Product Overview:	Recombinant full-length human CDK8/CCNC was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description:	CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for β-catenin-driven transformation and for expression of several β-catenin transcriptional targets.
Source:	Insect cell
Species:	Human
Tag :	GST
Form:	50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
Molecular Mass:	CDK8 ~84kDa and CCNC ~55 kDa
Purity:	The purity of CDK8/CCNC was determined to be >70% by densitometry.
Applications:	WB
Stability:	1 year at -70 centigrade
Storage:	Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:	0.1 μg/μL

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Gene Name:	cyclin dependent kinase 8 [ Homo sapiens (human) ]; cyclin C [ Homo sapiens (human) ]
Official Symbol:	CDK8; CCNC
Synonyms:	CDK8: K35, MGC126074, MGC126075; CyclinC; CCNC; CycC
Gene ID:	1024; 892
mRNA Refseq:	NM_001260; NM_005190
Protein Refseq:	NP_001251; NP_005181.2
MIM:	603184; 123838
UniProt ID:	P49336; P24863

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