Recombinant Human DNAJA1 Protein, His-tagged

• Cat.No.: DNAJA1-2169H
• Product Overview: Recombinant Human DNAJA1 Protein (Thr5-Leu234) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Thr5-Leu234
• Description: This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.2 kDa
  Accurate Molecular Mass: 32 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: DNAJA1 DnaJ (Hsp40) homolog, subfamily A, member 1 [ Homo sapiens (human) ]
• Official Symbol: DNAJA1
• Synonyms: DNAJA1; DnaJ (Hsp40) homolog, subfamily A, member 1; HSJ2; dnaJ homolog subfamily A member 1; dj 2; hdj 2; HSPF4; NEDD7; neural precursor cell expressed; developmentally down regulated 7; HSJ-2; human DnaJ protein 2; heat shock protein J2; dnaJ protein homolog 2; heat shock 40 kDa protein 4; heat shock protein, DNAJ-like 2; neural precursor cell expressed, developmentally down-regulated 7; DJ-2; DjA1; HDJ2; HSDJ; hDJ-2
• Gene ID: 3301
• mRNA Refseq: NM_001539
• Protein Refseq: NP_001530
• MIM: 602837
• UniProt ID: P31689