Recombinant Human ENPP2 protein, His-tagged
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| Cat.No. : | ENPP2-63H |
| Product Overview : | Recombinant Human ENPP2 protein (Asp49-Ile863) was fused to His-tag at the N-terminus and expressed in human 293 cells (HEK293). |
| Description : | Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. |
| Source : | HEK293 |
| Species : | Human |
| Tag : | His |
| Predicted N Terminal : | His |
| Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5, 10% trehalose. |
| Molecular Mass : | The protein has a calculated MW of 94.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE). |
| Protein length : | Asp49-Ile863 |
| Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
| Purity : | >95% as determined by SDS-PAGE. |
| Storage : | For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| SDS-PAGE of ENPP2-63H : |
Human ENPP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. |
| Gene Name : | ENPP2 |
| Official Symbol : | ENPP2 |
| Synonyms : | ENPP-2,E-NPP 2,Autotaxin,LysoPLD,ATX,PDNP2,ATX-X,NPP2,PD-IALPHA |
| Gene ID : | 5168 |
| mRNA Refseq : | NM_006209.5 |
| Protein Refseq : | NP_006200.3 |
| MIM : | 601060 |
| UniProt ID : | Q13822 |
◆ Recombinant Protein
| ENPP2-013H | Recombinant Human ENPP2 Protein, His-tagged | +Inquiry |
| ENPP2-567H | Recombinant Human ENPP2 Protein, His-tagged | +Inquiry |
| Enpp2-1190M | Recombinant Mouse Enpp2 Protein, His-tagged | +Inquiry |
| Enpp2-553R | Recombinant Rat Enpp2 Protein, His-tagged | +Inquiry |
| ENPP2-552H | Recombinant Human ENPP2 Protein, His-tagged | +Inquiry |
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◆ Lysates
| ENPP2-1939MCL | Recombinant Mouse ENPP2 cell lysate | +Inquiry |
| ENPP2-1556HCL | Recombinant Human ENPP2 cell lysate | +Inquiry |
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