Recombinant Human ENPP2 protein, His-tagged ENPP2-63H

Recombinant Human ENPP2 protein, His-tagged

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Recombinant Human ENPP2 protein, His-tagged

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Cat.No. : ENPP2-63H
Product Overview : Recombinant Human ENPP2 protein (Asp49-Ile863) was fused to His-tag at the N-terminus and expressed in human 293 cells (HEK293).
Description : Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : His
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 94.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Asp49-Ile863
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
SDS-PAGE of ENPP2-63H :

Human ENPP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : ENPP2
Official Symbol : ENPP2
Synonyms : ENPP-2,E-NPP 2,Autotaxin,LysoPLD,ATX,PDNP2,ATX-X,NPP2,PD-IALPHA
Gene ID : 5168
mRNA Refseq : NM_006209.5
Protein Refseq : NP_006200.3
MIM : 601060
UniProt ID : Q13822

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