Active Recombinant Human EPHA5 Protein, GST-tagged

• Cat.No.: EPHA5-3389H
• Product Overview: Human EPHA5 (NP_004430.3, 662 a.a. - 948 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells.

Specification

• Description: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
  Substrate: Blk/Lyntide.
  ATP: 100 μM.
• Molecular Mass: 59 kDa
• Purity: 89 % by SDS-PAGE/CBB staining.
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: EPHA5 EPH receptor A5 [ Homo sapiens ]
• Official Symbol: EPHA5
• Synonyms: EPHA5; EPH receptor A5; EphA5; ephrin type-A receptor 5; CEK7; EHK1; Hek7; TYRO4; EK7; EHK-1; EPH-like kinase 7; EPH homology kinase 1; Eph homology kinase-1; brain-specific kinase; receptor protein-tyrosine kinase HEK7; tyrosine-protein kinase receptor EHK-1; HEK7
• Gene ID: 2044
• mRNA Refseq: NM_004439
• Protein Refseq: NP_004430
• MIM: 600004
• UniProt ID: P54756