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Active Recombinant Human ERBB2 protein, His-tagged

Cat.No. : ERBB2-119H
Product Overview : Recombinant Human ERBB2 protein(Thr 23 - Ala 510), fused with His tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human Her2 (23-510), His Tag at 1 μg/mL (100 μL/well) can bind Pertuzumab Biosimilar with a linear range of 0.1-4 ng/mL.Immobilized Human Her2 (23-510), His Tag at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (Her2 × Her3) with a linear range of 0.2-3 ng/mL.
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 55.6 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Thr23-Ala510
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
Official Symbol : ERBB2
Synonyms : ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); NGL, v erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); receptor tyrosine-protein kinase erbB-2; CD340; HER 2; HER2; NEU; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); NGL; TKR1; HER-2; MLN 19; HER-2/neu;
Gene ID : 2064
mRNA Refseq : NM_001005862
Protein Refseq : NP_001005862
MIM : 164870
UniProt ID : P04626

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (8)

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Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate? 02/01/2023

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.

What is the primary role of ERBB2 in cell signaling? 08/12/2022

ERBB2 is a receptor tyrosine kinase involved in the regulation of cell growth and differentiation.

What therapeutic agents target ERBB2 in cancer treatment? 10/03/2020

Therapeutic agents targeting ERBB2 include trastuzumab and lapatinib.

In which types of cancer is ERBB2 commonly overexpressed? 05/12/2020

ERBB2 is commonly overexpressed in breast, ovarian, and stomach cancers.

How does ERBB2 overexpression affect patient prognosis in cancer? 05/04/2019

ERBB2 overexpression is often associated with aggressive disease and poor prognosis.

How can ERBB2 levels be assessed in a clinical setting? 08/27/2018

ERBB2 levels can be assessed using immunohistochemistry and fluorescence in situ hybridization (FISH) in tissue samples.

How is ERBB2 involved in cell growth and proliferation? 08/07/2018

It forms heterodimers with other ERBB receptors, activating pathways that promote cell growth and survival.

Are there known genetic mutations that lead to ERBB2 overexpression? 02/22/2018

Amplification of the ERBB2 gene leads to its overexpression in cancer cells.

Customer Reviews (3)

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Reviews
05/09/2022

    Worked as expected, satisfied.

    03/16/2022

      Reliable in protein assays.

      09/27/2020

        Quick and reliable shipping.

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