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Active Recombinant Human FCGR1A Protein, His & Avi-tagged, Biotinylated

Cat.No. : FCGR1A-373H
Product Overview : Recombinant Human FCGR1A Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 288 (Accession # P12314-1). Predicted N-terminus: Gln 16. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 34.3 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Specification
  • Gene Information
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Source : HEK293
Species : Human
Tag : His & Avi
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Yervoy Ipilumimab at 5 μg/mL (100 μL/well) can bind Recombinant Human FCGR1A Protein, His & Avi-tagged, Biotinylated with a linear range of 0.2-3 ng/mL (QC tested).
Recombinant Human FCGR1A Protein, His & Avi-tagged, Biotinylated captured on Biotin CAP- Series S Sensor Chip can bind Herceptin with an affinity constant of 1.02 nM as determined in a SPR assay (Biacore T200) (QC tested).
Molecular Mass : 34.3 kDa
Protein length : Gln 16 - Pro 288
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by reduced SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : FCGR1A Fc fragment of IgG, high affinity Ia, receptor (CD64) [ Homo sapiens ]
Official Symbol : FCGR1A
Synonyms : FCGR1A; CD64; CD64A; Fc-gamma RI; fc-gamma RIA; FCRI; IGFR1; FLJ18345;
Gene ID : 2209
mRNA Refseq : NM_000566
Protein Refseq : NP_000557
MIM : 146760
UniProt ID : P12314

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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