| Description : |
Fibroblast Growth Factor-6 (FGF-6) is a cytokine belonging to the heparin-binding FGF family, and is structurally related to other members of FGF family, particularly FGF-4. In vivo, FGF-6 exhibits an expression profile predominantly restricted tothe myogenic lineage, and it preferentially binds to two of the FGF receptors: FGFR1 and FGFR4. FGF-6 functions in muscle regeneration, myoblast proliferation and migration, and muscle differentiation in a dose-dependent manner. In vivo high concentration of recombinant FGF-6 up-regulates and down-regulates FGFR1 and FGFR4, respectively, as FGFR1 promotes the proliferation while FGFR4 promotes the differentiation in the muscle. Besides its dual function in muscle regeneration, FGF-6 may act as a regulator of bone metabolism as well. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence 1 μg/mL heparin, corresponding to a specific activity of > 4 × 10^5 units/mg. |
| Molecular Mass : |
18.8 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
169 |
| AA Sequence : |
MGTRANNTLLDSRGWGTLLSRSRAG LAGEIAGVNWESGYLVGIKRQRRLY CNVGIGFHLQVLPDGRISGTHEENP YSLLEISTVERGVVSLFGVRSALFV AMNSKGRLYATPSFQEECKFRETLL PNNYNAYESDLYQGTYIALSKYGRV KRGSKVSPIMTVTHFLPRI |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant human Fibroblast Growth Factor-6 (rhFGF-6) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhFGF-6 remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |
